African swine fever virus (ASFV) is a large, structurally complex double-stranded DNA virus with a 180–190 kbp genome encoding over 150 genes. It causes African swine fever (ASF) with mortality rates reaching 90–100% and has become a major threat to global pig production. The virus spread from sub-Saharan Africa to Eurasia after 2007 and was detected in the Dominican Republic and Haiti in 2021, marking the emergence of the Eurasian pandemic genotype in the Western Hemisphere.

Feline coronaviruses (FCoVs) exist primarily as two serotypes: Serotype-1 (FCoV1) and Serotype-2 (FCoV2). FCoV1 is more prevalent but difficult to culture than FCoV2, though both can mutate into the lethal Feline Infectious Peritonitis Virus (FIPV). The study addresses the lack of effective vaccines that cross-protect against FCoV1, as current commercial options are based on the easier to culture FCoV2. By leveraging comparative virology, the authors explore a "minimized" pan

This research falls under the broader scientific discipline of molecular biology and cancer therapeutics, specifically within RNA-guided genome editing and precision oncology. CRISPR-Cas13d systems represent a powerful class of RNA-targeting tools that can precisely edit target transcripts with minimal off-target effects. However, their clinical development faces significant challenges. One of these is the limited biostability of conventional linear guide RNAs (lgRNAs) suscep

This research falls under the broader scientific discipline of molecular biology and cancer therapeutics, specifically within RNA-guided genome editing and precision oncology. CRISPR-Cas13d systems represent a powerful class of RNA-targeting tools that can precisely edit target transcripts with minimal off-target effects. However, their clinical development faces significant challenges. One of these is the limited biostability of conventional linear guide RNAs (lgRNAs) suscep

This research falls under the broader scientific discipline of molecular biology and cancer therapeutics, specifically within RNA-guided genome editing and precision oncology. CRISPR-Cas13d systems represent a powerful class of RNA-targeting tools that can precisely edit target transcripts with minimal off-target effects. However, their clinical development faces significant challenges. One of these is the limited biostability of conventional linear guide RNAs (lgRNAs) suscep

Hepatocellular carcinoma (HCC) represents the second leading cause of cancer-related mortality worldwide, claiming over 700,000 lives annually. The liver's central role in blood filtration and metabolism makes it an attractive target for drug delivery, with lipid nanoparticles (LNPs) showing particular promise due to their natural hepatic tropism. However, LNP efficacy becomes compromised in HCC environments due to inadequate understanding of liver physiology under cancerous

Maternally derived antibodies serve as crucial early-life protection for piglets against infectious diseases, including swine influenza A virus (SwIAV). However, these same protective antibodies create significant challenges for vaccination programs by interfering with vaccine-induced immune responses, thereby reducing vaccine effectiveness. This interference has been well-documented with whole inactivated virus (WIV) vaccines against SwIAV, creating an urgent need for innova

This research falls under the broader scientific discipline of molecular biology and cancer therapeutics, specifically within RNA-guided genome editing and precision oncology. CRISPR-Cas13d systems represent a powerful class of RNA-targeting tools that can precisely edit target transcripts with minimal off-target effects. However, their clinical development faces significant challenges. One of these is the limited biostability of conventional linear guide RNAs (lgRNAs) suscep

This research falls under the broader scientific discipline of molecular biology and cancer therapeutics, specifically within RNA-guided genome editing and precision oncology. CRISPR-Cas13d systems represent a powerful class of RNA-targeting tools that can precisely edit target transcripts with minimal off-target effects. However, their clinical development faces significant challenges. One of these is the limited biostability of conventional linear guide RNAs (lgRNAs) suscep

Nagoya University: New cyclic disulfide lipids deliver mRNA five times more effectively for cancer treatment University of Ottawa: Development of nano-sized delivery agents with therapeutic potential beyond messenger function University of Pennsylvania: Chemical modification of ionizable lipids reduces inflammation and boosts mRNA therapeutic effectiveness Academic Journals: Multiple publications on circular guide RNA engineering, CRISPR-Cas13d systems, and advanced LNP ch

This research falls within the rapidly evolving field of cancer immunotherapy and nanomedicine, specifically focusing on cytokine-based therapeutic approaches. The study addresses a critical challenge in IL-12 cytokine therapy - the immunosuppressive feedback mechanisms that limit its therapeutic efficacy1. IL-12 has demonstrated potent antitumor activity in preclinical models by promoting T helper type 1 (TH1) differentiation and enhancing cytotoxic T lymphocyte activity. Ho

Schematic to explain a dual nanocarrier strategy for enhancing PDAC Tumor Immunity. The schematic illustrates a dual vaccination strategy, hypothesized to enhance the PDAC tumor immunity cycle by integrating endogenous and exogenous vaccination approaches. This strategy employs two distinct nanocarriers to synergistically reinforce CTL activation against PDAC. Endogenous Vaccination: Chemotherapeutic agents such as irinotecan induce ICD, triggering the release of tumor antige

We're proud to share that the Mayo Clinic Florida’s new RNA Research Facility has chosen the PreciGenome NanoGenerator™ Flex-S as their...

Efficient and precise delivery of CRISPR/Cas9 components remains one of the critical challenges toward the advancement of gene-editing therapies. Here, we have developed a high-throughput Barcode-Integration Nanoparticle Screen (BINS) method to evaluate a library of 96 lipid nanoparticles (LNPs) for their delivery efficiency across  in vitro  and  in vivo  models.  

This paper contributes to the field of RNA vaccinology, specifically addressing a key challenge in self-amplifying RNA (saRNA) vaccine development. While saRNA vaccines offer advantages over conventional mRNA platforms by providing longer-lasting protection at lower doses, they also trigger undesirable side effects at protective doses. These side effects stem from excessive innate immune activation driven by double-stranded RNA intermediates generated during self-replication.

This study by Nguyen and colleagues advances the field of veterinary vaccinology, specifically targeting African swine fever virus (ASFV), a highly contagious pathogen responsible for catastrophic losses in global swine populations. ASFV’s complex structure and antigenic diversity have hindered vaccine development, with prior efforts focusing on subunit vaccines targeting immunodominant proteins like p54, a structural antigen critical for viral entry and immune evasion. The p

This paper falls within the fields of medical mycology, immunology, and vaccinology, specifically addressing the emerging application of mRN

DOTMA vs. DOTAP lipid nanoparticles for GFP expression on human PBMCs and THP-1 cell line Authors:  Erwin Pavel Lamparelli, Elena...

This paper sits at the intersection of pharmaceutical technology, nanomedicine, and RNA therapeutics – fields that have gained tremendous at

Microfluidic Monodispersed Microbubble Generation Droplet