LipidFlex™ Lipid Nanoparticles Kits
for LNP Formulation
LipidFlex™ is a 3-component lipid mixture formulation, which can be directly used to generate liposome for small molecule encapsulation. In addition of different catonic/ionizable lipids, LipidFlex™ can also be used to generate lipid nanoparticles (LNPs) for encapsulation of small molecules and nucleic acid such as mRNA, DNA, protein, etc.
LipidFlex™ T cell kit includes LipidFlex T lipid mix and formulation buffers for high efficient mRNA LNP formulation for T cell transfection.
Flexible cationic/ionizable lipid ratio
Flexible with various N/P ratio
Size: 40-200 nm synthesis
Encapsulation Efficiency (E.E.%): >80% (>95-99% w. optimized conditions)
LipidFlex™ Experiment data:
LipidFlex™ T Cell Kit
LipidFlex™ T Cell Kit is a lipid nanoparticle (LNP) reagent mix. It is a highly efficient lipid formulation used to synthesize mRNA LNP for optimized gene delivery to primary human T cells. Using the NanoGenerator™ Flex-S system and CHIP-MIX-4 cartridge, customers can conveniently prepare potent mRNA LNP with high efficiency.
LipidFlex™ T Cell Kit Performance:
Narrow size distribution of mRNA LNP
High cell viability
High transfection efficiency
High protein expression level
Time efficient synthesis process
LipidFlex™ T Cell Kit Experiment data:
Flow cytometry result showing transfected human T cell using eGFP mRNA LNP.
The graph on the left show the non treated cells (negative control). On the right the treated cells show over 95% of the live human T-cells have been transfected and expressed eGFP in 24 hours.
With PreciGenomes LipidFlex™ T cell kit, the mean fluorescence intensity increased at least 200 fold (blue) compared to the negative control (red).
Protein expression level is dependent on the mRNA LNP dose. This can be seen as the mean fluorescence intensity increases with mRNA dose in T cells.
Over 90% of the cells are alive after the treatment which corresponds to the negative control in human T-cells and Jurkat cells.