Digital PCR (dPCR) Systems
Digital PCR (dPCR) is a quantitative PCR method, which offers the capacity of measuring the amount of DNA or RNA sensitively and accurately present in a sample. In order to measure the number of molecules in a sample with high precision, the sample is divided into a large number of partitions for reactions, which are analyzed separately. Some of these reactions contain the target molecule (positive) while others do not (negative).
DropDx digital PCR provides an accurate and sensitive method to detect and count individual nucleic acid molecules, enabling nucleic acid quantification with superior accuracy, precision, and relative sensitivity.
DropDx digital PCR system can be incredibly useful in applications of copy number variation, rare sequence detection, gene expression analysis, miRNA analysis, next-generation sequencing sample quantification, quantitation of low abundant genes or small differences, and rare event detection, such as cancer gene mutations. As absolute concentrations are determined, data can be easily interpreted and compared across different samples and experiments, especially in a clinical setting. Meanwhile, digital PCR is a simple and reproducible method that does not rely on a calibration curve for sample target quantification. Therefore no reference standards or endogenous controls are needed.
System Features & Benefits
Multiple fluorescent channels
Low cost solutions
Integrated Software (No PC required)
With integrated digital PCR cartridge, DropDx system enables rapid achievement of Poisson distribution of samples in the droplet preparation process, which utilizes patented microfluidic technology and a unique reagent system. It is fully automated with one-button operation. The station incorporates patented temperature control components for top performance, along with the temperature control management system. With the fully integrated cartridge, it achieves the amplification of nucleic acid samples.
Automatic work flow
Runtime ~2.5 hours from sample to results
Digital PCR Assay Kits
FastPlex Digital PCR assays are designed by experts in the digital PCR field. Every type of the assays has been experimentally validated on DropDx platform to ensure optimal performance for its target application. Meanwhile, with PCR conditions optimization these assays also could be used on other popular digital PCR platforms.
These predesigned assays for digital PCR come with a FAM, HEX, VIC, ROX, or Cy5 Fluorophore. Other Fluorophores are also available.
FastPlex PCR assays have been widely used in experiments for applications including:
Rare mutation and sequence detection
Copy number variation analysis
Calibration of standards
Analysis of inhibitory samples
Splice variant preference
Next-generation sequencing library analysis
Cis vs trans localization
PreciGenome also offers the service for dPCR assay design and validation. Our scientist will work with you to design digital PCR Assay Design Service assays based on your specific requirements. All validation data are provided to clients. The sequence information is also available with some extra fee.
We also have the service to convert your qPCR assay to digital PCR assay.