Recirculating Perfusion System

Cell Culture under Flow

Recirculating perfusion system is a cell culture device with a bioreactor is connected to a recirculation pathway, where the exchange of spent media occurs.

PreciGenome Recirculating Perfusion System offers the capacity of simulation of various physiological conditions, such as continuous unidirectional, oscillating, and pulsatile flow. It allows control over microenvironmental cues, such as cell-cell and cell-matrix interactions; the potential to scale experiments; the use of small culture volumes; and the ability to integrate with microsystem technologies for on-chip experimentation.

The perfusion systems also has minimal mechanical stress, minimal amount of medium and supplement. It allows precise and on-demand delivery of biochemical reagent in the extracellular microenvironment, and controlled application of mechanical forces exerted via fluid flow.

Drug screening
Organ-On-a-Chip
Calcium imaging
Bioreactor research
Mimicking shear stress conditions in arterial flow

Single-Reagent Delivery

Applications:

Long-term culture with defined shear stress
Cell response to medium change
Live cell imaging and immunofluorescence for shear stress response analysis
3D cell culture
Stem cells assays

Option #1

Combining our PG-MF controller with a 2-positions/6-ports valve, PreciGenome provides a perfusion system dedicated to cell culture in perfusion chambers with controlled shear force. This fully integrated solution includes all the necessary elements (hardware and software) to create a continuous and recirculated (unidirectional or bidirectional) flow and controlled flow rate (shear force) applied onto cells.

PG-MFC controller is used to provide pressure to pump reagent from reservoir 1 through liver-heart-on-a-chip to reservoir 2 through the valve. Combining PG-MFC pressure controller with one 2-positions/6-ports valve ( extra two 3-way valves required for light version controller), the system allows flowing buffers in two separate reservoirs back and forth, but still keeps the flow in the microfluidic chip uni-directional.

System Benefits:

Controlled shear stress
Long duration experiments
Automation
Controllable flow rates
Ready to connect with the incubator
Temperature control module is also available to integrate into the system

Work Modes:

Unidirectional flow control
Bidirectional flow control
Pulse flow control

Option #1 Recirculation Perfusion System with PG-MFC Controller

microfluidic recirculation system diagram, organ on a chip, cell culture, perfusion system, precigenome

Example of microfluidic recirculation system setup for the organ-on-a-chip application.

Unidirectional reagent flow is able to run through the chip over days.

As showing in the schematics below, in position 1, reagent flows out from reservoir 1, through the 2 positions/6 ports valve, enters the microfluidic chip from its left side, and flows into reservoir 2. In this position, the 3-way valve on the left side connects pressure source to reservoir 1 and the other 3-way valve connects to the atmosphere.

In position 2, reagent flows out from reservoir 2, enters the microfluidic chip from its left side, and flows into reservoir 1. In this position, the 3-way valve on the right side connects pressure source to reservoir 2 and the other 3-way valve connects to the atmosphere. In this application, only a single pressure source is needed.