PLA/PLGA nanocarriers have emerged as promising vehicles for drug delivery, offering controlled release and targeted therapeutic capabilities. This study demonstrates a novel approach using microfluidics-assisted nanoprecipitation with the PreciGenome Nanogenerator Flex system to fabricate fluorescent and gold-loaded nanocarriers.
Methodology and Fabrication
The researchers employed a microfluidics-assisted nanoprecipitation technique using the PreciGenome Nanogenerator Flex instrument to produce well-defined PLA/PLGA nanocarriers. The process involved precise control of flow rates and mixing conditions, resulting in uniform particle size distribution.
Key Findings
Nanocarrier Characteristics
The fabricated nanocarriers exhibited consistent physicochemical properties with controlled size distributions. Rhodamine-loaded particles demonstrated excellent fluorescent properties, while gold-loaded carriers showed distinctive optical characteristics.
Cellular Uptake and Distribution
The study revealed efficient cellular internalization of both fluorescent and gold-loaded nanocarriers. Tracking experiments confirmed successful cellular uptake and distribution, with the particles showing optimal intracellular targeting capabilities.
Performance Metrics
Uniform size distribution with controlled particle morphology
High encapsulation efficiency for both Rhodamine and gold payloads
Sustained release profiles under physiological conditions
Minimal cytotoxicity in tested cell lines
Technical Advantages
The microfluidics-assisted approach using the PreciGenome system offered several advantages over conventional methods:
Precise control over particle formation
Reproducible batch-to-batch consistency
Scalable production capability
Enhanced loading efficiency
Implications and Applications
This research demonstrates the potential of microfluidics-based PLA/PLGA nanocarrier systems for various biomedical applications, including:
Drug delivery
Cellular tracking
Diagnostic imaging
Therapeutic monitoring
The successful implementation of the PreciGenome Nanogenerator Flex system for nanocarrier synthesis represents a significant advancement in the field of controlled drug delivery systems, offering a reliable platform for the development of next-generation therapeutic carriers.
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PLA/PLGA nanocarriers fabricated by microfluidics-assisted nanoprecipitation and loaded with Rhodamine or gold can be efficiently used to track their cellular uptake and distribution