Plant-derived phytosterols — β-sitosterol, stigmasterol, and fucosterol — as cholesterol substitutes in mRNA-LNPs improve transfection efficiency and lyophilization stability. This 2026 Materials Today Bio study demonstrates functional restoration of vision in RCS rats via MERTK mRNA gene therapy, with LNPs formulated using the PreciGenome NanoGenerator®.

Efficient and precise delivery of CRISPR/Cas9 components remains one of the critical challenges toward the advancement of gene-editing therapies. Here, we have developed a high-throughput Barcode-Integration Nanoparticle Screen (BINS) method to evaluate a library of 96 lipid nanoparticles (LNPs) for their delivery efficiency across in vitro and in vivo models.