This research addresses a critical gap in understanding how RNA-binding proteins (RBPs) contribute to osteoarthritis pathogenesis and explores the therapeutic potential of protein replacement therapy using innovative circular RNA delivery systems. This offers new perspectives on treating degenerative joint diseases through precision molecular interventions.

This research falls under the broader scientific discipline of molecular biology and cancer therapeutics, specifically within RNA-guided genome editing and precision oncology. CRISPR-Cas13d systems represent a powerful class of RNA-targeting tools that can precisely edit target transcripts with minimal off-target effects. However, their clinical development faces significant challenges. One of these is the limited biostability of conventional linear guide RNAs (lgRNAs) suscep