LipidFlex™ Lipid Nanoparticles Kits
for LNP Formulation

Lipid nanoparticles (LNPs) are formulated by the mixing of two phases, an ethanol phase and an aqueous phase. The ethanol phase is a four-component ethanol phase containing ionizable lipid, helper phospholipid, cholesterol, and lipid-anchored PEG. The aqueous phase is an acidic aqueous phase containing payloads such as mRNA, DNA, small molecules, etc. (Learn more about LNP formulation)

LipidFlex™ is a 3-component lipid mixture formulation, which can be directly used to generate liposome for small molecule encapsulation. In addition of different catonic/ionizable lipids, LipidFlex™ can also be used to generate lipid nanoparticles (LNPs) for encapsulation of small molecules and nucleic acid such as mRNA, DNA, protein, etc.

LipidFlex™ RNA kit includes LipidFlex lipid mix and formulation buffers for high efficient LNP formulation for mRNA, siRNA, circRNA, etc.

LipidFlex™ T cell kit includes LipidFlex lipid mix and formulation buffers for high efficient mRNA LNP formulation for T cell transfection.

Narrow size distribution of mRNA LNP

High cell viability

High transfection efficiency

High protein expression level

Custom design available

LipidFlex™ RNA Kit

LipidFlex™ RNA kit includes LipidFlex lipid mix and formulation buffers for high efficient LNP formulation for mRNA, siRNA, circRNA, etc.

nanoparticle synthesis, liposome, DNA encapsulation, LNP, lipid nanoparticle, Lipid NP, nanogenerator, precigenome, mRNA LNP, drug delivery, precision medicine , flex, pro, formulation, research

Features:

LipidFlex™ RNA Kit is a lipid nanoparticle (LNP) reagent mix. It is a highly efficient lipid formulation used to formulate LNP with RNA (mRNA, siRNA, circRNA, etc.) encapsulation for optimized gene delivery.

Using the NanoGenerator™ Flex-S system and CHIP-MIX-4 cartridge, customers can conveniently prepare potent mRNA LNP with high efficiency.

LipidFlex™ Experiment data:

Size: 40-200 nm synthesis
PDI: 0.05-0.2
Encapsulation Efficiency (E.E.%): >80% (>95-99% w. optimized conditions)

LipidFlex Kit

LipidFlex™ T Cell Kit

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LipidFlex™ T cell kit brochure

Order LipidFlex™ T cell kit

Features:

LipidFlex™ T Cell Kit is a lipid nanoparticle (LNP) reagent mix. It is a highly efficient lipid formulation used to synthesize mRNA LNP for optimized gene delivery to primary human T cells. Using the NanoGenerator™ Flex-S system and CHIP-MIX-4 cartridge, customers can conveniently prepare potent mRNA LNP with high efficiency.

LipidFlex™ T Cell Kit Experiment data:

GFP+ 0.003%

GFP+ 96.8%

nanoparticle synthesis, LNP, lipid nanoparticle, Lipid NP, nanogenerator, precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, precision nanosystems, nanoassemblr, t cell kit, GenVoy

Negative control

Flow cytometry result showing transfected human T cell using eGFP mRNA LNP.

The graph on the left show the non treated cells (negative control). On the right the treated cells show over 95% of the live human T-cells have been transfected and expressed eGFP in 24 hours.

With PreciGenomes LipidFlex™ T cell kit, the mean fluorescence intensity increased at least 200 fold (blue) compared to the negative control (red).

Protein expression level is dependent on the mRNA LNP dose. This can be seen as the mean fluorescence intensity increases with mRNA dose in T cells.

Over 90% of the cells are alive after the treatment which corresponds to the negative control in human T-cells and Jurkat cells.

LipidFlex™ 3-lipid Mix

LipidFlex™ is a 3-component lipid mixture formulation, which can be directly used to generate liposome for small molecule encapsulation. In addition of different catonic/ionizable lipids, LipidFlex™ can also be used to generate lipid nanoparticles (LNPs) for encapsulation of small molecules and nucleic acid such as mRNA, DNA, protein, etc.