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Organ-on-a-Chip System

Organ-on-a-Chip technology provides a novel in vitro platform with a possibility of reproducing physiological functions of in vivo tissue, more accurately than conventional cell-based model systems.

 

The technology opens up great opportunities for next-generation experiments of mimicking human organ functionality, microphysiology and morphology in vitro, replacing traditional animal-based model systems.

 

By realizing different organ functions on a chip, organ-on-a-chip technology is potentially useful for building models of complex diseases. The technology can also be used to study pharmacokentic model when new drugs are being developed.

 

Organ-on-a-Chip System with Pressure Controller

System Overview:

PG-MFC controller provides both vacuum and pressure and makes it perfectly suited to organ-on-a-chip applications.

 

Combining the controller with a peristaltic pump, the system provides reagent recirculation capability to ensure cell nurturing over days.

 

Here we provide two examples of organ-on-a-chip applications.

  1. The first example is lung-on-a-chip. It utilizes both pressure and vacuum our controller can provide.

  2. The second example is a recirculating system between liver and heart built from our controller.

System Setup Example #1

- Lung-on-a-Chip

PG-MFC controller has multiple sources of vacuum and pressure. It meets most requirements of organ-on-a-chip applications.

In this lung-on-a-chip case, two pressure and one vacuum sources are used which connect to different inlets of a chip. Two pressure lines push different reagents to deliver into the chip to mimic blood flow into lung and exchange chemicals through cell channel bilayer. The vacuum line connects to the side chamber to simulate the breathing process in a lung.

Example of sine output on vacuum Line, which is used to actuate the membrane between the top and bottom chambers. 
Meanwhile, the airflow rate is also monitored in realtime to indicate the leakage in the flow.

System Setup Example #2

- Liver-Heart-on-a-Chip

In this example, cell lines are used to mimic heart and livers on a microfluidic chip with reagent recirculation.

 

PG-MFC controller is used to provide pressure to pump reagent from reservoir 1 through liver-heart-on-a-chip to reservoir 2 through a 2 positions/6 ports valve. Combining PG-MFC pressure controller with one 2 positions/6 ports valve and two 3-way valves, the system allows flowing buffers in two separate reservoirs back and forth, but still keeps the flow in the microfluidic chip uni-directional.

Example of microfluidic recirculation system setup for the organ-on-a-chip application. 
Unidirectional reagent flow is able to run through the chip over days.

As showing in the schematics above, in position 1, reagent flows out from reservoir 1, through the 2 positions/6 ports valve, enters the microfluidic chip from its left side, and flows into reservoir 2. In this position, the 3-way valve on the left side connects pressure source to reservoir 1 and the other 3-way valve connects to the atmosphere.

 

In position 2, reagent flows out from reservoir 2, enters the microfluidic chip from its left side, and flows into reservoir 1. In this position, the 3-way valve on the right side connects pressure source to reservoir 2 and the other 3-way valve connects to the atmosphere. In this application, only a single pressure source is needed.

Smart peristaltic pump is designed to meet your fluid transfer and dispensing needs with superior precision and accuracy, easy operation and minimum maintenance. The ultra-bright 4.3-inch LCD touch screen control panel interactively guides you at each step of the operation to set up sophisticated dispensing procedures in just seconds. In 4 different operation modes: Continuous Dispense Mode, Volume Dispense Mode, Repeat Dispense Mode, and Schedule Dispense Mode, the human-machine interface (HMI) accepts direct process parameter input and displays readout of rotor speed, flow rate, dispensed volume, remaining time or elapsed time data all on one screen.

Organ-on-a-Chip System with Peristaltic Pump

System Benefits:​

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  • Long duration experiments

  • Automation

  • Controllable flow rates

  • Ready to connect with the incubator

  • Cost-effective