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lipid nanoparticle, lipid nanoparticles, Lipid NP,  LNP, LNP preparation, LNP formulation, lipid nanoparticle synthesis, liposome, mRNA encapsulation,   precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, nanogenerator, flex-s, flex-m, pro, max, precisionnanosystems, pni, NanoAssemblr, ignite, spark
Lipid nanoparticle LNP

NanoGenerator™ Nanoparticle Synthesis System
Preclinical Formulation Development & Optimization

lipid nanoparticle, lipid nanoparticles, Lipid NP,  LNP, LNP preparation, LNP formulation, lipid nanoparticle synthesis, liposome, mRNA encapsulation,   precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, nanogenerator, flex-s, flex-m, pro, max, precisionnanosystems, pni, NanoAssemblr, ignite, spark
lipid nanoparticle, lipid nanoparticles, Lipid NP,  LNP, LNP preparation, LNP formulation, lipid nanoparticle synthesis, liposome, mRNA encapsulation,   precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, nanogenerator, flex-s, flex-m, pro, max, precisionnanosystems, pni, NanoAssemblr, ignite, spark
lipid nanoparticle, lipid nanoparticles, Lipid NP,  LNP, LNP preparation, LNP formulation, lipid nanoparticle synthesis, liposome, mRNA encapsulation,   precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, nanogenerator, flex-s, flex-m, pro, max, precisionnanosystems, pni, NanoAssemblr, ignite, spark

Scalable throughput from microLiters to Liters

lipid nanoparticle, lipid nanoparticles, Lipid NP,  LNP, LNP preparation, LNP formulation, lipid nanoparticle synthesis, liposome, mRNA encapsulation,   precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, nanogenerator, flex-s, flex-m, pro, max, precisionnanosystems, pni, NanoAssemblr, ignite, spark
PreciGenome NanoGenerator™ is a high-performance instrument for nanoparticle synthesis such as lipid nanoparticles(LNP), liposomes, PLGA, etc. NanoGenerator™ generates nanoparticles with better uniformity in size and smaller PDI. It is scalable from 0.1 mL/sample screening up to 1L (>10L with custom design) large volume GMP production

Nanoparticles, especially lipid nanoparticles, have shown great potential for biomedical applications in a variety of fields such as pharmaceutical industries like drug delivery, mRNA vaccines, and biosensing because of its excellent properties. The lipid nanoparticles
, liposomes, and PLGA  are the most commonly used biodegradable materials for the delivery of both hydrophilic and hydrophobic compounds. 
 
Nanoparticle synthesis by microfluidic technology presents advantages over the conventional batch synthesis processes due to its capacity in better uniformity in size and shape. For example, in the drug delivery field, a variety of nanoparticles such as LNP, liposomes, and PLGA can be synthesized by using NanoGenerator™ nanoparticle synthesis system.

Principle of Particle Synthesis

The NanoGenerator™ platform employs microfluidic devices for controllable and tunable nanoparticle production. 
lipid nanoparticle, lipid nanoparticles, Lipid NP,  LNP, LNP preparation, LNP formulation, lipid nanoparticle synthesis, liposome, mRNA encapsulation,   precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, nanogenerator, flex-s, flex-m, pro, max, precisionnanosystems, pni, NanoAssemblr, ignite, spark

The schematic above illustrates a microfluidic channel with micro-mixing structures for nanoparticle synthesis. The system leverages precise manipulation of fluids (aqueous phase and solvent phase) at the microscale, achieving fast, controlled  and highly efficient mixing processes of different solution phases.

 System Benefits

High Performance & Efficiency
  • Tunable size (40-200nm)

  • Low PDI

  • High encapsulation efficiency

Automation & QC
  • Automated workflow

  • Real-time data monitoring & recording

Open Platform
  • LNP reagents

  • Microfluidic mixer chips

Cost Effective
  • Affordable system

  • Low cost consumables

  • Minimum maintenance

Scalable & Reproducible
  • Scale-up and minimum process development

  • Direct transfer from clinical development to commercial manufacturing

  • Reproducible manufacturing

Custom Design & Service
  • Custom design & OEM

  • Research collaboration 

  • On-site 3Q installation & qualification (optional)

System Benefits

Path from Discovery to Commercialization

lipid nanoparticle, lipid nanoparticles, Lipid NP,  LNP, LNP preparation, LNP formulation, lipid nanoparticle synthesis, liposome, mRNA encapsulation,   precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, nanogenerator, flex-s, flex-m, pro, max, precisionnanosystems, pni, NanoAssemblr, ignite, spark

The NanoGenerator is a fully equipped instrument, ready for immediate use in the development of preclinical nanomedicine drugs. It offers controllable and reproducible mixing conditions, ensuring the accurate synthesis of LNPs through its scalable architecture found in the entire NanoGenerator product line.

NanoGenerator system offers controllable and reproducible mixing conditions, ensuring the accurate synthesis of LNPs through its scalable architecture found in the entire NanoGenerator product line. This architecture facilitates the seamless transfer of crucial process parameters, guaranteeing consistent critical quality attributes (CQAs) as one transitions from discovery and screening, process development to GMP production and commercial manufacturing for a wide range of applications, such as mRNA LNP vaccine development.

Scalable and Reproducible Formulation Development

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NanoGenerator™ platform ensures consistent critical quality attributes (such as particle size, PDI, encapsulation efficiency, etc.) throughout the entire development and manufacturing process. This guarantees speed, cost-effectiveness, and reliability at every stage.

Advantages of Microfluidic Method

Payloads

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mRNA/siRNA

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Proteins and peptides

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DNA

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Small molecule drugs

NanoGenerator Flex-S

​NanoGenerator™ Flex-S System facilitates the rapid screening of nanoparticle formulations and early-stage mRNA candidates, offering a substantial increase in project efficiency.  It offers precise control of parameters ensures consistent critical quality attributes, guaranteeing speed, cost-effectiveness, and reliability at every stage. 

Flex-S is designed for small scale production. The throughput volume range is from 0.1 to 0.5 mL, which is perfect for formulation screening and early discovery applications. Smaller output volume (<0.05ml per run) is achievable by recipe optimization. With smaller output volume, the reagents cost including payload and carrier can reduced more than 80% per run.

NanoGenerator Flex-S

NanoGenerator Flex-M

lipid nanoparticle, lipid nanoparticles, Lipid NP,  LNP, LNP preparation, LNP formulation, lipid nanoparticle synthesis, liposome, mRNA encapsulation,   precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, nanogenerator, flex-s, flex-m, pro, max, precisionnanosystems, pni, NanoAssemblr, ignite, spark

NanoGenerator Flex-M

NanoGenerator™ applies microfluidic approaches to synthesize nanoparticles (LNP, liposome, PLGA, etc.) in a continuous mode. NanoGenerator™ Flex-M system provides a wide throughput range from 1 to 12 mL, which meets a variety of applications from early screening to animal studies.

Flex-M system also provides an in-line dilution option to reduce ethanol concentration instantly. It further stabilizes LNP products.
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NanoGenerator PRO

lipid nanoparticle, lipid nanoparticles, Lipid NP,  LNP, LNP preparation, LNP formulation, lipid nanoparticle synthesis, liposome, mRNA encapsulation,   precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, nanogenerator, flex-s, flex-m, pro, max, precisionnanosystems, pni, NanoAssemblr, ignite, spark

NanoGenerator PRO

NanoGenerator™ Pro is an integrated nanoparticle synthesis system.

NanoGenerator™ Pro contains more powerful pressure control modules, which provide higher throughput from 2 to 200mL. The total flow rate range is from 4 to 20mL/min. The flow rate ratio (W:O) is between 2:1 to 5:1.
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NanoGenerator Flex-M
NanoGenerator PRO

NanoGenerator MAX

lipid nanoparticle, lipid nanoparticles, Lipid NP,  LNP, LNP preparation, LNP formulation, lipid nanoparticle synthesis, liposome, mRNA encapsulation,   precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, nanogenerator, flex-s, flex-m, pro, max, precisionnanosystems, pni, NanoAssemblr, ignite, spark

NanoGenerator MAX (RUO version)
large scale production

NanoGenerator™ Max RUO version is widely used for preclinical application for lipid nanoparticle synthesis.

With PreciGenome microfluidics technology, customers can easily and seamlessly transfer their early discovery results (NanoGenerator Flex-S, Flex-M, and Pro) to late stage production (NanoGenerator Max cGMP).
max scalable.jpg
MAX specs and data.png
NanoGenerator™ Max cGMP version is aslo available. It is designed for the clinical and commercial production.

 
NanoGenerator MAX

Microfluidic Mixer Chips

PreciGenome offers a variety of microfluidic chips in different materials to meet most of our customers' application requirements for nanoparticles synthesis.
 
Microfluidic mixer chips are usually for nanoparticles synthesis. Three types of materials, including polymers, glass and silicon, are commonly used to fabricate microfluidic chips. Material of the chip is selected depending on the application requirements, including chip design, types of solvent or reagent used for experiment, needs of the application, budget, and fabrication time,etc. 

LipidFlex™ LNP Formulation

LipidFlex™ is a 3-component lipid mixture, which can be directly used to generate liposome for small molecule encapsulation. In addition of different catonic/ionizable lipids, LipidFlex™ can also be used to generate lipid nanoparticles (LNPs) for encapsulation of small molecules and nucleic acid such as mRNA, DNA, protein, etc.

LipidFlex™ T cell kit includes LipidFlex T lipid mix and formulation buffers for high efficient mRNA LNP formulation for T cell transfection.

Features: 
  • Flexible cationic/ionizable lipid ratio

  • Flexible with various N/P ratio

  • Size: 80-120 nm  synthesis 

  • PDI: 0.05-0.2 

  • Encapsulation Efficiency (E.E.%): >90% (95-99% w. optimized conditions)

nanoparticle synthesis, liposome, DNA encapsulation, LNP, lipid nanoparticle, Lipid NP, nanogenerator, precigenome, mRNA LNP, drug delivery, precision medicine , flex, pro, formulation, research
LipidFlex LNP Reagents
Synthesis Service

Synthesis Service

  • Formulation design  (Lipid NP, Liposome or PLGA)

Customize liposomes design based on our clients’ demand by varying lipid compositions, vesicle sizes, surface charges, etc.

  • Payload encapsulation 

Customize protocols to encapsulate drug molecules into lipid nanoparticle or PLGA with high encapsulation efficiency.

  • Cell study 

Cell in vitro transfection service.

  • Analysis and characterization

Run comprehensive analysis assays for liposomes before and after encapsulation, which includes visual appearance, size distribution, stability, entrapment efficiency, encapsulation efficiency analysis,  in vitro release profile analysis, release rate, etc.

 

Applications

System Applications

Gene Therapy

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Genetic drugs, including small interfering RNA (siRNA), mRNA, or plasmid DNA, hold promise for treating a wide array of diseases by either suppressing harmful genes, producing therapeutic proteins, or employing gene-editing techniques. Currently, lipid nanoparticle (LNP) systems stand at the forefront among non-viral delivery methods, enabling the clinical utilization of genetic drugs.

Vaccines

Preventive services

Lipid nanoparticle has played a pivotal role in expediting the development of vaccines, as demonstrated by Pfizer and Moderna in the COVID-19 pandemic response. It allows for precise and high-throughput screening of potential vaccine candidates. The technology facilitates rapid testing, antigen formulation, and optimization of vaccine delivery systems.

Cell Therapy

cell therapy.jpg

Cell Therapies entail the transformation of cells, commonly immune cells, obtained either from the patient (autologous) or a compatible donor (allogeneic). These modified cells undergo isolation, amplification, and are later reintroduced into the patient. Lipid Nanoparticles (LNPs) present a versatile approach to cell reprogramming, facilitating the transfer of RNA responsible for protein expression or gene editing. 

Other Areas

liposome cosmetics.jpg

Other applications including cosmetics, medical imaging, nutrition, agrochemicals, etc.
The cosmetics sector stood at the forefront in acknowledging and utilizing nanotechnology advancements in diverse product innovations. Liposomal cosmetic formulations are expected to offer several benefits, including improved stability and effectiveness, along with successful ingredient penetration into the skin. A variety of liposomal cosmetics are currently in use.

Demo Videos and Webinars

NanoGenerator Flex-M Demo Video 

NanoGenerator LNP Synthesis & Application Webinar with ProMab Biotechnologies

Resources

Lipid Nanoparticle and Liposome Synthesis

Application

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Precise lipid NP and liposome synthesis by microfluidic mixing methods. Efficient method for generating nanoparticles for targeted drug or nutrient delivery.

Vaccines 2023
 

Publication

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A Single-Dose Intramuscular Immunization of Pigs with Lipid Nanoparticle DNA Vaccines Based on the Hemagglutinin Antigen Confers Complete Protection against Challenge Infection with the Homologous Influenza Virus Strain

Lipid Nanoparticle & Liposome Introduction

Article

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Introduction of lipid nanoparticle and  liposome. It introduces the structure of LNP, LNP formulation, and LNP preparation protocol, etc.

International Journal of Pharmaceutics 2022

Publication

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Lipid nano-vesicles for thyroid hormone encapsulation: A comparison between different fabrication technologies, drug loading, and an in vitro delivery to human tendon stem/progenitor cells in 2D and 3D culture

PLGA Nanoparticle Synthesis by NanoGenerator

Application

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Drug-loaded PLGA nanoparticles (NPs) can be synthesized by our system. PLGA NPs synthesis in continuous flow microfluidics by the nanoprecipitation process.

Int. J. Mol. Sci. 2023
 

Publication

Promab Screenshot 2023-10-31 111843.png

CAR-NK Cells Generated with mRNA-LNPs Kill Tumor Target Cells In Vitro and In Vivo

Related Products
Document & Inquiry

Documents

  • NanoGenerator brochure
  • NanoGenerator technical presentation
  • Application notes for lipid nanoparticle synthesis
  • Case study: T cell mRNA transfection 

Some of Our Customers

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