NanoGenerator® Nanoparticle Synthesis System
Preclinical Formulation Development & Optimization
Scalable throughput from microLiters to Liters
NanoGenerator® is a high-performance nanoparticle synthesis system for formulation and preparation of nanoparticles, including lipid nanoparticles (LNPs), liposomes, PLGA, etc. NanoGenerator produces nanoparticles with improved size uniformity and lower polydispersity index (PDI). It offers scalable production, ranging from ultra-low volumes of 0.1 mL for early-stage screening to large-scale batch production of hundreds of liters, enabling seamless, continuous GMP-compliant manufacturing.
Nanoparticles, especially lipid nanoparticles, have shown great potential for several biomedical applications, including drug delivery, mRNA vaccines, and biosensing. Lipid nanoparticles, liposomes, and PLGA nanoparticles are the most commonly used biodegradable materials for the delivery of both hydrophilic and hydrophobic compounds. All these types can be synthesized using the NanoGenerator platform.
Principle of Particle Synthesis
The NanoGenerator platform employs microfluidic devices for controllable and tunable nanoparticle production.
The schematic above illustrates a microfluidic channel with micro-mixing structures for nanoparticle synthesis. The system leverages precise manipulation of fluids (aqueous phase and solvent phase) at the microscale, achieving fast, controlled, and highly efficient mixing.
System Benefits
High Performance & Efficiency
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Tunable size (40-200nm)
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Low PDI
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High encapsulation efficiency
Cost Effective
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Affordable system
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Low cost consumables
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NO limit on the mixer usage
Mixer Compatibility
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Microfluidic mixer chips
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T-mixer
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Impinging jet mixer (IJM)
Automation & QC
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Automated workflow
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Real-time data monitoring & recording
Scalable & Reproducible
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Scale-up and minimum process development
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Direct transfer from discovery to commercial manufacturing
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Reproducible manufacturing
Custom Design & Service
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Custom design & OEM
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Research collaboration
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On-site 3Q installation & qualification (optional)
Path from Discovery to Commercialization
NanoGenerator is suitable for the development of pre-clinical nanomedicine drugs. It offers controllable and reproducible mixing conditions, ensuring the accurate synthesis of LNPs through the scalable architecture found in its entire product line.
This architecture facilitates the seamless transfer of crucial process parameters, guaranteeing consistent critical quality attributes (CQAs) as one transitions from discovery and screening, to process development, to GMP production and commercial manufacturing for a wide range of applications. Examples include mRNA LNP vaccine development, gene therapy, cell therapy etc.
Scalable and Reproducible Formulation Development
NanoGenerator ensures consistent critical quality attributes (such as particle size, PDI, encapsulation efficiency, etc.) throughout the entire development and manufacturing process. This guarantees speed, cost-effectiveness, and reliability at every stage.
Advantages of Microfluidic Synthesis
Payloads
mRNA/siRNA
Proteins and peptides
DNA
Small molecule drugs
Instrument
NanoGenerator Flex-S
early discovery & screening
NanoGenerator Flex-M & M Premium
NanoGenerator Flex-M
NanoGenerator's pre-clinical lineup applies microfluidics to synthesize nanoparticles with continuous liquid flow rate control. The NanoGenerator Flex-M provides a wide throughput range from 1 to 12 mL, which meets a variety of applications from early screening to small animal studies.
NanoGenerator Flex-M Premium
The NanoGenerator Flex-M Premium provides a wider throughput range from 1 to 200 mL, which meets a variety of applications from early screening to animal studies.
Both system also provides an in-line dilution option to reduce ethanol concentration instantly. This further stabilizes LNP products and may be combined with post-processing purification.
NanoGenerator MAX
NanoGenerator MAX (RUO version)
large scale production
The NanoGenerator Max RUO may be used for pre-clinical lipid nanoparticle synthesis. With PreciGenome's microfluidic technology, users can easily and seamlessly transfer their early discovery results (NanoGenerator Flex-S or Flex-M) to late stage production (NanoGenerator Max cGMP).
Microfluidic Mixer Chips
Three types of materials, including polymers, glass and silicon, are commonly used to fabricate microfluidic chips. Material of the chip is selected depending on the application requirements, including chip design, types of solvent or reagent used for experiment, needs of the application, budget, and fabrication time,etc.
PreciGenome offers a variety of microfluidic chips in different materials to meet most of our customers' requirements. Most are made of Topas COC, though options made of glass or PEEK are also available.
System Applications
Gene Therapy
Genetic drugs, including small interfering RNA (siRNA), mRNA, and plasmid DNA, hold promise for treating a wide array of diseases by either suppressing harmful genes, producing therapeutic proteins, or employing gene-editing techniques. Currently, LNP systems stand at the forefront among non-viral delivery methods, enabling the clinical utilization of genetic drugs.
Cell Therapy
Cell therapy entails the transformation of cells, commonly immune cells, obtained from either the patient (autologous) or a compatible donor (allogeneic). These modified cells undergo isolation and amplification. They are then reintroduced into the patient. Lipid nanoparticles present a versatile approach to cell reprogramming, facilitating the transfer of RNA responsible for protein expression or gene editing.
Vaccines
Lipid nanoparticles have played a pivotal role in expediting the development of vaccines, as demonstrated by Pfizer and Moderna in the COVID-19 pandemic response. It allows for precise and high-throughput screening of potential vaccine candidates. The technology facilitates rapid testing, antigen formulation, and optimization of vaccine delivery systems.
Other Areas
Other LNP applications include cosmetics, medical imaging, nutrition, agrochemicals, etc.The cosmetics sector stood at the forefront in acknowledging and utilizing nanotechnology advancements in diverse product innovations. Liposomal cosmetic formulations are expected to offer several benefits, including improved stability and effectiveness, along with successful ingredient penetration into the skin. A variety of liposomal cosmetics are currently in use.
Demo Videos and Webinars
NanoGenerator Flex-S Demo Video
NanoGenerator LNP Synthesis & Application Webinar with ProMab Biotechnologies
Documents
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NanoGenerator brochure
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NanoGenerator technical presentation
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Application notes for lipid nanoparticle synthesis
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Case study: T cell mRNA transfection
Some of Our Customers
