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LipidFlex™ Lipid Nanoparticles (LNP) and Liposomes Formulation 

LipidFlex™ is a 3-component lipid mixture formulation, which can be directly used to generate liposome for small molecule encapsulation. In addition of different catonic/ionizable lipids, LipidFlex™ can also be used to generate lipid nanoparticles (LNPs) for encapsulation of small molecules and nucleic acid such as mRNA, DNA, protein, etc.

LipidFlex™ T cell kit includes LipidFlex T lipid mix and formulation buffers for high efficient mRNA LNP formulation for T cell transfection.
nanoparticle synthesis, liposome, DNA encapsulation, LNP, lipid nanoparticle, Lipid NP, nanogenerator, precigenome, mRNA LNP, drug delivery, precision medicine , flex, pro, formulation, research

LipidFlex 

  • Flexible cationic/ionizable lipid ratio

  • Flexible with various N/P ratio

  • Size: 40-200 nm  synthesis 

  • PDI: 0.05-0.2 

  • Encapsulation Efficiency (E.E.%): >80% (>95-99% w. optimized conditions)

 Features:

nanoparticle synthesis, liposome, DNA encapsulation, LNP, lipid nanoparticle, Lipid NP, nanogenerator, precigenome, mRNA LNP, drug delivery, precision medicine , flex, pro, formulation, research
  • LipidFlex brochure

LipidFlex Experiment data:

LipidFlex Data.jpg
LipidFlex

LipidFlex™ T Cell Kit 

Features:

LipidFlex T Cell Kit is a lipid nanoparticle (LNP) reagent mix. It is a highly efficient lipid formulation used to synthesize mRNA LNP for optimized gene delivery to primary human T cells. Using the NanoGenerator Flex-S system and CHIP-MIX-4 cartridge, customers can conveniently prepare potent mRNA LNP with high efficiency. 

T cell, nanoparticle synthesis, liposome, DNA encapsulation, LNP, lipid nanoparticle, Lipid NP, nanogenerator, precigenome, mRNA LNP, drug delivery, precision medicine , flex, pro, formulation, research
  • LipidFlex T cell kit brochure

LipidFlex™ T Cell Kit Performance:

Screenshot 2023-03-17 105325.png
Narrow size distribution
of mRNA LNP
Screenshot 2023-03-17 105340.png
High transfection efficiency
High protein expression level
High cell viability
Time efficient synthesis process

LipidFlex™ T Cell Kit Experiment data:

nanoparticle synthesis, LNP, lipid nanoparticle, Lipid NP, nanogenerator, precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, precision nanosystems, nanoassemblr, t cell kit, GenVoy

GFP+ 0.003%

GFP+ 96.8%

Negative control

Flow cytometry result showing transfected human T cell using eGFP mRNA LNP.

The graph on the left show the non treated cells (negative control). On the right the treated cells show over 95% of the live human T-cells have been transfected and expressed eGFP in 24 hours.

nanoparticle synthesis, LNP, lipid nanoparticle, Lipid NP, nanogenerator, precigenome, mRNA LNP, PLGA, drug delivery, precision medicine, precision nanosystems, nanoassemblr, t cell kit, GenVoy

With PreciGenomes LipidFlex T cell kit, the mean fluorescence intensity increased at least 200 fold (blue) compared to the negative control (red). 

tcell data final g3.png

Protein expression level is dependent on the mRNA LNP dose. This can be seen as the mean fluorescence intensity increases with mRNA dose in T cells.

tcell data final g4.png

Over 90% of the cells are alive after the treatment which corresponds to the negative control in human T-cells and Jurkat cells. 

T Cell Kit

Lipid Nanoparticles Synthesis System
     for Process & Manufacturing 

Lipid nanoparticles (LNPs) or solid lipid nanoparticles (SLNs, sLNPs) are nanoparticles composed of lipids and possess a solid lipid core matrix which is able to solubilize lipophilic molecules. Typically they are spherical with an average diameter between 10 and 1000 nanometers.

Lipid nanoparticles have emerged across the pharmaceutical industry as promising vehicles to deliver a variety of therapeutics.  They are a novel pharmaceutical drug delivery system and formulation. LNPs became more widely known in late 2020, as some COVID-19 vaccines that use RNA vaccine technology coat the fragile mRNA strands with PEGylated lipid nanoparticles as their delivery vehicle.

Liposomes, an early version of LNPs, are a versatile nanomedicine delivery platform. A number of liposomal drugs have been approved and applied to medical practice. A liposome is a spherical vesicle with at least one lipid bilayer. The liposome can be used as a vehicle for administration of nutrients and pharmaceutical drugs. 
Liposomes are composite structures, consisting of phospholipids, especially phosphatidylcholine. It may contain small amounts of other molecules. Though liposomes can vary in size from low micrometer range to tens of micrometers, unilamellar liposomes, shown as the Figure below, are typically in the lower size range with various targeting ligands. The ligands are attached to their surface allowing for their surface-attachment and accumulation in pathological areas for treatment of disease.


 
PreciGenome_nanoG plex, nanogenerator, flex, nanoparticle synthesis, drug delivery, mRNA, lipid NP, liposome, PLGA

Nanoparticle Systhesis:
 
Lipid stream is squeezed into a narrow stream flow parallel each other to two adjacent aqueous streams flowing at higher flow rates along the central channel, enables precise nanoprecipitation through the rapid mixing and spontaneous diffusion process

PRO, research, nanoparticle synthesis, liposome, DNA encapsulation, LNP, lipid nanoparticle, Lipid NP, nanogenerator, precigenome, mRNA LNP, PLGA, drug delivery, precision medicine , flex
research, nanoparticle synthesis, liposome, DNA encapsulation, LNP, lipid nanoparticle, Lipid NP, nanogenerator, precigenome, mRNA LNP, PLGA, drug delivery, precision medicine , flex

NanoGenerator PRO

NanoGenerator Flex

LipidFlex™ 

LipidFlex™ is a 3-component lipid mixture.

LipidFlex™ can be directly used to generate liposome for small molecule encapsulation.

In addition of different catonic/ionizable lipids, LipidFlex™ can also be used to generate lipid nanoparticles (LNPs) for nucleic acid encapsulation.

Features:
 
  • Flexible cationic/ionizable lipid ratio

  • Flexible with various N/P ratio

  • Size: 80-120 nm  synthesis 

  • PDI: 0.05-0.2 

  • Encapsulation Efficiency (E.E.%): >90% (>95-99% w. optimized conditions)

LipidFlex-1by5ml.jpg
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