SpCas9 mRNA (ARCA, 5mCTP, ψUTP)
 
This SpCas9 mRNA encodes an optimized version of the Streptococcus pyogenes Cas9 nuclease. Used together with a purified guide RNA (gRNA), it directs site-specific recognition and cleavage of target genomic DNA within the Type II CRISPR-Cas genome editing system. The encoded Cas9 protein carries two nuclear localization signals (NLS) and a C-terminal HA epitope tag, which support efficient nuclear delivery, a higher rate of DNA cleavage, and easier downstream purification.
 
Designed for efficient in vitro transfection, PreciGenome’s SpCas9 mRNA provides a high-quality, ready-to-use alternative to plasmid- or protein-based Cas9 delivery. Its ARCA-capped, poly(A)-tailed, nucleotide-modified design supports high translation efficacy while minimizing innate immune activation — a research-grade tool for CRISPR-Cas9 genome editing, gene knockout, and functional gene-editing studies.
 
Product Features
ARCA (anti-reverse cap analog) capping for high translation efficacy.
5-Methyl-CTP and Pseudo-UTP (ψUTP) modified nucleotides to reduce innate immune activation and enhance mRNA stability.
Poly(A) tail for stable expression.
Dual nuclear localization signals (NLS) plus a C-terminal HA epitope tag for efficient nuclear delivery and downstream purification.
 
Product Specifications
Form: Liquid
Concentration: 1.0–1.4 mg/mL
Buffer: 1 mM Sodium Citrate, pH 6.4
Storage: Store at –40 °C or below.
Shelf Life: 2 years from manufacturing date
 
Quality Control
Appearance: Colorless liquid
RNA Purity: ≥ 95% (agarose gel electrophoresis)
OD260/OD280: 1.70–2.30
Endotoxin: < 10 EU/mg
pH: 6.0–7.0
For research use only.

Contact us for custom formulations or special requests.
 
Sourced from APExBIO, Catalog No. R1006.