Recirculating Perfusion System 

Cell Culture under Flow

Recirculating perfusion system is a cell culture device with a bioreactor is connected to a recirculation pathway, where the exchange of spent media occurs.
 
PreciGenome Recirculating Perfusion System offers the capacity of simulation of various physiological conditions, such as continuous unidirectional, oscillating, and pulsatile flow. It allows control over microenvironmental cues, such as cell-cell and cell-matrix interactions; the potential to scale experiments; the use of small culture volumes; and the ability to integrate with microsystem technologies for on-chip experimentation.
 
The perfusion systems also has minimal mechanical stress, minimal amount of medium and supplement. It allows precise and on-demand delivery of biochemical reagent in the extracellular microenvironment, and controlled application of mechanical forces exerted via fluid flow.

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  • Drug screening

  • Organ-On-a-Chip

  • Calcium imaging

  • Bioreactor research

  • Mimicking shear stress conditions in arterial flow

 
 

Applications:

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  • Long-term culture with defined shear stress 

  • Cell response to medium change

  • Live cell imaging and immunofluorescence for shear stress response analysis

  • 3D cell culture

  • Stem cells assays

Combining our PG-MF controller with a 2-positions/6-ports valve, PreciGenome provides a perfusion system dedicated to cell culture in perfusion chambers with controlled shear force. This fully integrated solution includes all the necessary elements (hardware and software) to create a continuous and recirculated (unidirectional or bidirectional) flow and controlled flow rate (shear force) applied onto cells.

PG-MFC controller is used to provide pressure to pump reagent from reservoir 1 through liver-heart-on-a-chip to reservoir 2 through the valve. Combining PG-MFC pressure controller with one 2-positions/6-ports valve ( extra two 3-way valves required for light version controller), the system allows flowing buffers in two separate reservoirs back and forth, but still keeps the flow in the microfluidic chip uni-directional.

System Benefits:​

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  • Controlled shear stress

  • Long duration experiments

  • Automation

  • Controllable flow rates

  • Ready to connect with the incubator

  • Temperature control module is also available to integrate into the system

Work Modes:​

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  • Unidirectional flow control

  • Bidirectional flow control

  • Pulse flow control

Option #1 Recirculation Perfusion System with PG-MFC Controller 

Example of microfluidic recirculation system setup for the organ-on-a-chip application. 
Unidirectional reagent flow is able to run through the chip over days.

As showing in the schematics below, in position 1, reagent flows out from reservoir 1, through the 2 positions/6 ports valve, enters the microfluidic chip from its left side, and flows into reservoir 2. In this position, the 3-way valve on the left side connects pressure source to reservoir 1 and the other 3-way valve connects to the atmosphere.

 

In position 2, reagent flows out from reservoir 2, enters the microfluidic chip from its left side, and flows into reservoir 1. In this position, the 3-way valve on the right side connects pressure source to reservoir 2 and the other 3-way valve connects to the atmosphere. In this application, only a single pressure source is needed.

 System Content:​

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  • PG-MFC controller, 1 pc (4Channel , 8Channel or custom version available)

  • Bidirectional 6-port / 2-position valve,  1 pc

  • Reservoir Kit (15ml, 50ml or 100ml),  2 sets

  • Liquid flow sensor (optional)

  • Tubings and fittings, 1 set

 

Part #: PG-PF-4-REC

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Demo Video of Unidirectional Flow Mode

Perfusion controller
Flow unit with 1.5ml, 15ml, 50ml or 100ml reservoir kit options
Software for complex process control and real-time data monitoring
 

Option #2 Recirculation Perfusion System          with Light Version Controller

 System Content:​

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  • PG-MFC-LT-2CH controller, 1 pc 

  • Bidirectional 6-port / 2-position valve,  1 pc

  • 3-way valves, 2 pcs

  • Reservoir Kit (15ml, 50ml or 100ml),  2 sets

  • Liquid flow sensor (optional)

  • Tubings and fittings, 1 set

 

Part #: PG-PF-LT2-REC

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Microfluidic Perfusion Chips 

PreciGenome offers a variety of chips in different materials to meet most of our customers' application requirements.  Three types of materials, including polymers, glass and silicon, are commonly used to fabricate microfluidic chips. Material of the chip is selected depending on the application requirements, including chip design, types of solvent or reagent used for experiment, needs of the application, budget, and fabrication time,etc. 
 

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Precigenome LLC | 2176 Ringwood Ave. San Jose, CA, 95131, USA

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