CD3+ Cell Targeted Antibody-Conjugated Premade eGFP mRNA Lipid Nanoparticle
 
The CD3+ Cell Targeted Antibody-Conjugated Premade eGFP mRNA Lipid Nanoparticle represents a cutting-edge targeted delivery system designed for precise mRNA delivery to CD3+ T cells. This innovative formulation combines the specificity of anti-human CD3 antibody (OKT3) with the efficiency of lipid nanoparticle technology to achieve enhanced cellular targeting. The system features covalently conjugated CD3 antibodies on the surface of SM102-based lipid nanoparticles, loaded with fully modified eGFP mRNA containing N1-methyl-pseudouridine (m1Ψ) modifications for improved stability and reduced immunogenicity.
 
Key Features
Targeted Delivery: Surface-conjugated anti-human CD3 antibody (OKT3) mAb for specific targeting of CD3+ T cells
Enhanced mRNA Stability: eGFP mRNA with 100% U-base modification using N1-methyl-pseudouridine (m1Ψ)
Optimized Formulation: SM102-based ionizable lipid system for improved cellular uptake and endosomal escape
Covalent Conjugation: Antibody covalently linked to lipid molecules ensuring stable targeting functionality
Ready-to-Use: Premade formulation eliminates preparation time and ensures batch-to-batch consistency
Research-Grade Quality: Manufactured under controlled conditions for reliable experimental results
 
Applications
T Cell Research: Studying CD3+ T cell biology, activation, and function
Immunotherapy Development: Evaluating targeted delivery approaches for T cell-based therapies
Cell Tracking Studies: Real-time monitoring of T cell populations using eGFP expression
Proof-of-Concept Studies: Demonstrating targeted mRNA delivery efficiency to specific immune cell populations
Drug Development: Screening and optimization of T cell-targeted therapeutic approaches
Flow Cytometry Analysis: Quantitative assessment of targeting efficiency and expression levels
 
Product Specifications
Parameter
Specification
Payload
eGFP mRNA (m1Ψ)
Conjugated Antibody
Anti-human CD3 (OKT3) mAb
Ionizable Lipid
SM102
Formulation Components
Ionizable lipid, DSPC, PEG-2000 DMG, Cholesterol
Storage Buffer
10% Sucrose in TBS
Appearance
Clear and colorless
Concentration
Batch specific (~0.1 mg/mL)
Availability
On-demand
 
Storage & Handling Instructions
Storage Conditions:
Store at -20°C or below
Minimize freeze-thaw cycles to preserve LNP integrity
Protect from light during storage and handling
Shipping:
Ships on dry ice to maintain product stability
Handling Recommendations:
Avoid repeated freeze-thaw cycles
Thaw gently at room temperature or 4°C
Mix gently by inversion; avoid vortexing
Allow to equilibrate to working temperature before use
 
Custom Solutions
At PreciGenome, we understand that research requirements vary. We offer comprehensive customization options for this product:
Alternative Targeting Antibodies: Custom antibody conjugation for different cell surface markers
Payload Modifications: Alternative mRNA sequences, reporters, or therapeutic genes
Formulation Adjustments: Modified lipid compositions or concentrations
Scale Options: Various volume and concentration specifications
Buffer Systems: Alternative storage and working buffers
Bulk Manufacturing: Large-scale production for extensive studies
Our technical team works closely with researchers to develop tailored solutions that meet specific experimental needs and research objectives.
 
Important Notes
For Research Use Only. This product is not for human or veterinary use.
For technical support, custom formulations, or bulk orders, please contact us.
This product contains genetically modified materials and should be handled according to institutional biosafety guidelines
Performance may vary depending on cell type, experimental conditions, and handling procedures
Optimal transfection conditions should be determined empirically for each specific application
Product specifications are subject to batch-specific variations; refer to individual Certificate of Analysis
Users should validate targeting efficiency and expression levels in their specific experimental systems