saCas9 mRNA (Cap1, m1Ψ)
 
This saCas9 mRNA encodes a wild-type Staphylococcus aureus Cas9 (saCas9) enzymatic protein. Used together with a purified guide RNA (gRNA), it directs site-specific recognition and cleavage of target genomic DNA for CRISPR-Cas9 gene editing in cells.
 
Designed for efficient in vitro transfection, PreciGenome's saCas9 mRNA provides a high-quality, ready-to-use alternative to plasmid- or protein-based Cas9 delivery. Its Cap1-capped, poly(A)-tailed, m1Ψ-modified design supports high translation efficacy while minimizing innate immune activation — a research-grade tool for CRISPR-Cas9 genome editing, gene knockout, and functional gene-editing studies.
 
Product Features
Cap1 structure for high capping efficiency.
100% substitution with N1-methylpseudouridine (m1Ψ) for improved protein expression and reduced innate immune response.
Optimized untranslated regions (UTRs) to enhance translation.
Poly(A) tail to mimic a natural mature mRNA.
 
Product Specifications
Form: Liquid
Concentration: 1 mg/mL
mRNA Length: 3534 nucleotides
Molecular Weight: 1.14 × 10⁶ Da
Buffer: 1 mM Sodium Citrate, pH 6.5
Storage: Store at –20 °C for short term (<3 months); –80 °C for long term.
 
Quality Control
Appearance: Clear, free of foreign particles
RNA Content: Target ± 5%
Integrity: ≥ 75%
OD260/OD280: 1.70–2.30
Capping Efficiency: ≥ 90%
Endotoxin: < 10 EU/mg
pH: Target ± 0.5
For research use only.

Contact us for custom formulations or special requests.