Firefly Luciferase mRNA LNP Premade Lipid Nanoparticle
 
The Firefly Luciferase mRNA-LNP is an advanced lipid nanoparticle formulation containing firefly luciferase (FLuc) mRNA designed for efficient cellular delivery and protein expression studies. This ready-to-use product leverages cutting-edge lipid nanoparticle technology to facilitate transfection of encapsulated mRNA into mammalian cells and enable in vivo delivery applications. Upon cellular uptake and endosomal escape, the encapsulated FLuc mRNA is released and translated into functional luciferase protein, making it an ideal tool for bioluminescence-based research applications.
 
Key Features
High Encapsulation Efficiency: Optimized lipid composition ensures maximum mRNA loading and protection
Enhanced Cellular Uptake: Ionizable lipid formulation promotes efficient endosomal escape and cytoplasmic delivery
Modified mRNA: Contains pseudouridine-modified (m1Ψ) FLuc mRNA for improved stability and reduced immunogenicity
Versatile Delivery: Compatible with a wide range of mammalian cell lines, including hard-to-transfect cells
Ready-to-Use: Premade formulation eliminates preparation time and ensures consistent results
Multiple Lipid Options: Available with various ionizable lipids to suit specific research requirements
 
Applications
mRNA delivery efficiency studies
Protein translation and expression analysis
Cell viability and cytotoxicity assessments
Bioluminescence imaging and tracking
Transfection optimization protocols
In vitro and in vivo delivery research
Drug delivery system development
 
 
Product Specifications
Parameter
Specification
Payload
Firefly Luciferase mRNA (m1Ψ modified)
Formulation
Ionizable lipid, DSPC, PEG-2000 DMG, Cholesterol
Storage Buffer
10% Sucrose in TBS
Appearance
Clear and colorless
Concentration
Batch specific (~0.1 mg/mL)
Stock Availability
On-demand
Shipping Conditions
Dry ice
Available Ionizable Lipids                                                 
SM-102, DLin-MC3, ALC-0315, LP-01, C12-200, Lipid 5, Lipid A9, Lipid C2, Lipid 29, Lipid CL1, C14-02, C14-14, cKK-E12, 9322-O16B
 
Storage & Handling Instructions
Storage Temperature: -20°C or below
Handling Precautions: Avoid repeated freeze-thaw cycles to preserve LNP integrity
Thawing: Allow to thaw slowly at room temperature or 4°C before use
Mixing: Gently mix by pipetting; avoid vortexing which may damage particles
Aliquoting: Consider dividing into smaller aliquots to minimize freeze-thaw exposure
 
Custom Solutions
PreciGenome offers customized formulations to meet specific research requirements. Our custom services include:
Alternative ionizable lipid selections
Modified mRNA sequences
Different buffer systems
Specialized particle sizes
Bulk quantity preparations
Custom concentration ranges
For special formulations and other requirements, please contact our technical team.
 
Important Notes
For Research Use Only. This product is not for human or veterinary use.