Firefly Luciferase mRNA LNP Premade Lipid Nanoparticle

The Firefly Luciferase mRNA-LNP is an advanced lipid nanoparticle formulation containing firefly luciferase (FLuc) mRNA designed for efficient cellular delivery and protein expression studies. This ready-to-use product leverages cutting-edge lipid nanoparticle technology to facilitate transfection of encapsulated mRNA into mammalian cells and enable in vivo delivery applications. Upon cellular uptake and endosomal escape, the encapsulated FLuc mRNA is released and translated into functional luciferase protein, making it an ideal tool for bioluminescence-based research applications.

Key Features

• High Encapsulation Efficiency: Optimized lipid composition ensures maximum mRNA loading and protection

• Enhanced Cellular Uptake: Ionizable lipid formulation promotes efficient endosomal escape and cytoplasmic delivery

• Modified mRNA: Contains pseudouridine-modified (m1Ψ) FLuc mRNA for improved stability and reduced immunogenicity

• Versatile Delivery: Compatible with a wide range of mammalian cell lines, including hard-to-transfect cells

• Ready-to-Use: Premade formulation eliminates preparation time and ensures consistent results

• Multiple Lipid Options: Available with various ionizable lipids to suit specific research requirements

Applications

• mRNA delivery efficiency studies

• Protein translation and expression analysis

• Cell viability and cytotoxicity assessments

• Bioluminescence imaging and tracking

• Transfection optimization protocols

• In vitro and in vivo delivery research

• Drug delivery system development

Product Specifications

Storage & Handling Instructions

• Storage Temperature: -20°C or below

• Handling Precautions: Avoid repeated freeze-thaw cycles to preserve LNP integrity

• Thawing: Allow to thaw slowly at room temperature or 4°C before use

• Mixing: Gently mix by pipetting; avoid vortexing which may damage particles

• Aliquoting: Consider dividing into smaller aliquots to minimize freeze-thaw exposure

Custom Solutions

PreciGenome offers customized formulations to meet specific research requirements. Our custom services include:

• Alternative ionizable lipid selections

• Modified mRNA sequences

• Different buffer systems

• Specialized particle sizes

• Bulk quantity preparations

• Custom concentration ranges

For special formulations and other requirements, please contact our technical team.

Important Notes

For Research Use Only. This product is not for human or veterinary use.