EGFP mRNA-LNP w. Lipophilic Dye, Premade Lipid Nanoparticle

The EGFP mRNA-LNP w. Lipophilic Dye is an innovative premade lipid nanoparticle formulation designed for advanced research applications requiring both protein expression and real-time tracking capabilities. This dual-functional system combines EGFP mRNA encoding for red fluorescent protein expression with integrated lipophilic dyes, enabling researchers to effectively monitor and image intracellular trafficking and biodistribution of LNPs both in vitro and in vivo. The product offers exceptional versatility for studying LNP delivery mechanisms, cellular uptake pathways, and biodistribution patterns.

Key Features

Dual Functionality: Combines EGFP protein expression with lipophilic dye labeling for comprehensive tracking
Optimized mRNA: Features pseudouridine-modified (m1Ψ) EGFP mRNA for enhanced stability and reduced immunogenicity
Advanced Formulation: Professionally formulated with ionizable lipid, DSPC, PEG-2000 DMG, and cholesterol for optimal delivery
Multiple Dye Options: Available with four different lipophilic dyes covering diverse excitation/emission spectra
Flexible Ionizable Lipids: Choice of industry-standard ionizable lipids including SM-102, DLin-MC3, ALC-0315, and LP-01
Ready-to-Use: Premade formulation eliminates preparation time and ensures batch-to-batch consistency

Applications

Intracellular Trafficking Studies: Real-time monitoring of LNP uptake and intracellular distribution
Biodistribution Analysis: In vivo tracking of LNP delivery to target tissues and organs
Delivery Mechanism Research: Investigation of cellular uptake pathways and endosomal escape
Pharmacokinetic Studies: Assessment of LNP circulation time and clearance patterns
Tissue-Specific Delivery: Evaluation of targeting efficiency to specific cell types or tissues
Formulation Development: Comparative studies of different LNP formulations and their performance
Drug Delivery Optimization: Development and validation of therapeutic delivery strategies

Product Specifications

Parameter	Specification
Payload	EGFP mRNA (m1Ψ modified)
mRNA Concentration	~0.1 mg/mL (batch specific)
Dye Concentration	0.1 mole % of selected dye
Formulation Components	Lipophilic dye, Ionizable lipid, DSPC, PEG-2000 DMG, Cholesterol
Storage Buffer	10% Sucrose in TBS
Appearance	Clear and colorless
Available Dye Options	DiO (488/509 nm), DiI (549/565 nm), DiD (644/665 nm), DiR (750/780 nm)
Ionizable Lipid Options	SM-102, DLin-MC3, ALC-0315, LP-01
Stock Availability	On-demand production

Storage & Handling Instructions

Storage Conditions:

Store at -80°C or below for long-term stability
Minimize freeze-thaw cycles to preserve LNP integrity and functionality
Protect from light to maintain dye stability

Shipping:

Products are shipped on dry ice to maintain cold chain integrity

Handling Guidelines:

Avoid repeated freeze-thaw cycles
Allow product to thaw completely at room temperature before use
Gently mix by pipetting; avoid vortexing

Custom Solutions

We understand that research requirements vary, and we're committed to supporting your specific needs. Our custom solutions include:

Alternative Dye Selection: Additional lipophilic dyes beyond our standard options
Modified Concentrations: Adjusted mRNA or dye concentrations to meet experimental requirements
Alternative Ionizable Lipids: Custom lipid formulations for specialized applications
Different mRNA Payloads: Custom mRNA sequences for specific research needs
Bulk Quantities: Large-scale production for extensive studies
Specialized Formulations: Modified compositions for specific delivery targets

For custom formulations and specialized requirements, please contact our technical team to discuss your project needs.

Important Notes

For Research Use Only. This product is not for human or veterinary use.