NanoGenerator Nanoparticle Synthesis System

Nanoparticles, especially liposome and polymer nanoparticles, have shown great potential for biomedical applications in a variety of fields such as pharmaceutical industries like drug delivery, mRNA vaccines, and biosensing because of its excellent properties.
 
Nanoparticle synthesis by microfluidic technology presents advantages over the conventional batch synthesis processes due to its capacity in better uniformity in size and shape. For example, in the drug delivery field, a variety of nanoparticles such as lipid nanoparticles (LNP), liposomes, and PLGA can be synthesized by using NanoGenerator™ nanoparticle synthesis system. The lipid nanoparticles (LNP), liposomes, and PLGA  are the most commonly used biodegradable materials for the delivery of both hydrophilic and hydrophobic compounds. 
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NanoGenerator Pro

PreciGenome_nanoG plex, nanogenerator, flex, nanoparticle synthesis, drug delivery, mRNA, lipid NP, liposome, PLGA

NanoGenerator Flex

Principle of Particle Synthesis:

NanoGenerator™ nanoparticle synthesis system employs microfluidic device for controlled and tunable polymer particles’ production. The schematic below illustrates the device with junction in focused-flow geometry designed for particles’ synthesis. Solvent displacement methods are used for nanoparticles synthesis.
PreciGenome_nanoG plex, nanogenerator, flex, nanoparticle synthesis, drug delivery, mRNA, lipid NP, liposome, PLGA

Nanoparticle Systhesis:

 

Polymer stream is squeezed into a narrow stream flow parallel each other to two adjacent aqueous streams flowing at higher flow rates along the central channel, enables precise nanoprecipitation through the rapid mixing and spontaneous diffusion process

PreciGenome_nanoG plex, nanogenerator, flex, nanoparticle synthesis, drug delivery, mRNA, lipid NP, liposome, PLGA

System diagram for nanoparticle synthesis, such as PLGA, Liposome nanoparticles

 
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 System Benefits:​

  • Controllable particle size

  • Low DPI

  • High encapsulation efficiency

  • Standard connection. Easy connect to other components

  • OEM service and module integration available

PreciGenome_nanoG plex, nanogenerator, flex, nanoparticle synthesis, drug delivery, mRNA, lipid NP, liposome, PLGA
High Performance & Efficiency
  • Tunable size (40-500nm)
  • Low PDI
  • High encapsulation efficiency
Open Platform
  • Reagents
  • Microfludic chips
Scalable Throughput
  • Small volume test
  • High volume production
  • GMP version available soon.
Simple Operation
  • Easy setup
  • Intuitive UI w. touchscreen
Cost effective
  • Affordable configurations
  • Low cost solutions

Advantages of Microfluidic Method:

 

System Contents:

​​NanoGenerator™ Flex

Part #:  PG-SYN-8F           (with iFlow Touch controller)           

​​NanoGenerator™ Pro

  • Instrument, 1 pc

  • Reservoir kit,  50ml, 4 sets

  • Microfluidic mixer chips, 2 pcs 

  • Tubing and fittings, 1 set

  • Demo reagent kit for liposome synthesis 

Part #:  PG-SYN-P       

Microfluidic Mixer Chips:

PreciGenome offers a variety of microfluidic chips in different materials to meet most of our customers' application requirements for nanoparticles synthesis.
 
Microfluidic mixer chips are usually for nanoparticles synthesis. Three types of materials, including polymers, glass and silicon, are commonly used to fabricate microfluidic chips. Material of the chip is selected depending on the application requirements, including chip design, types of solvent or reagent used for experiment, needs of the application, budget, and fabrication time,etc. 
 

LipidFlex™ 

LipidFlex™ is a 3-component lipid mixture.

LipidFlex™ can be directly used to generate liposome for small molecule encapsulation.

In addition of different catonic/ionizable lipids, LipidFlex™ can also be used to generate lipid nanoparticles (LNPs) for nucleic acid encapsulation.

Features:
 
  • Flexible cationic/ionizable lipid ratio

  • Flexible with various N/P ratio

  • Size: 80-120 nm  synthesis 

  • PDI: 0.05-0.2 

  • Encapsulation Efficiency (E.E.%): >90% (>95-99% w. optimized conditions)

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Payloads:

  • DNA/mRNA/siRNA

  • Small molecule drugs

  • Proteins and peptides

  • Other payloads

Synthesis System Applications:

  • Drug Delivery (PLGA, Lipid NP & Liposome)

Droplet microfluidics enables useful platforms for drug delivery vehicles and drug molecules as novel functional materials. Because of uniform size, monodisperse size distribution, and desired properties, droplet microfluidics demonstrates promising potential for the production of complex drug systems.

 

  • Chemical synthesis

Droplet-based microfluidics has become an important method for chemical synthesis. Droplets are able to act as individual reactions, which are free from contamination from outside. Meanwhile, it also offers benefits of high throughput, small reagent volumes, low cost, portability, a high degree of synthetic control, rapid reaction, etc.

 

  • mRNA Liposome Vaccine

Liposomes and liposome-derived nanovesicles such as archaeosomes and virosomes have become important carrier systems in vaccine development. The liposome-based vaccine delivery systems has advantages in versatility and plasticity. 

 

  • Gel Particle Synthesis

In the last decade, the gel particles (hydrogels, microgels, and nanogels) has been an area of interest for many researchers and industries. Because of high throughput, mono-dispersity of particles, and low cost, droplet-based microfluidic systems have been widely used for synthesizing these hydrogel particles.

Applications Notes:

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PLGA Nanoparticle Synthesis

Drug-loaded PLGA nanoparticles (NPs) can be synthesized by our system. PLGA NPs synthesis in continuous flow microfluidics by the nanoprecipitation process.

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Lipid Nanoparticle and Liposome Synthesis

Precise lipid NP and liposome synthesis by microfluidic mixing methods. Efficient method for generating nanoparticles for targeted drug or nutrient delivery

Synthesis Service:

  • Formulation design  (Lipid NP, Liposome or PLGA)

Customize liposomes design based on our clients’ demand by varying lipid compositions, vesicle sizes, surface charges, etc.

  • Payload encapsulation 

Customize protocols to encapsulate drug molecules into lipid nanoparticle or PLGA with high encapsulation efficiency.

  • Cell study 

Cell in vitro transfection service.

  • Analysis and characterization

Run comprehensive analysis assays for liposomes before and after encapsulation, which includes visual appearance, size distribution, stability, entrapment efficiency, encapsulation efficiency analysis,  in vitro release profile analysis, release rate, etc.

 

 
 

Documents:

  • NanoGenerator brochure
  • NanoGenerator datasheet and application note

Some of Our Customers

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