Coronavirus (COVID-19) SARS-CoV-2 PCR Nucleic Acid Detection Kits

(qPCR and Digital PCR assays)

Coronaviruses are a class of RNA viruses with a capsule and a linear single-stranded positive genome, about 80-120 nm in diameter. Currently, they only infect human, mouse, pig, cat, dog, and avian vertebrates. The novel coronavirus (Covid-19) has been confirmed as a new variant that can cause viral pneumonia, mild fever and dry cough, severe breathing difficulties and even shock.

This kit is used for nucleic acid detection of new coronavirus (Covid-19), and the results can be used for auxiliary diagnosis of patients with new coronavirus infection or patients suspected of new coronavirus infection.

The test results of this kit are for RUO and should not be used as the sole standard for clinical diagnosis. It is recommended to conduct a comprehensive analysis of conditions in combination with the clinical manifestations of patients and other laboratory tests.



RT-qPCR 2019-Novel Coronavirus Assay

Detection Principle

This kit uses the 2019-Novel Coronavirus (Covid-19) ORF 1ab and the specific conserved sequence encoding the nucleocapsid protein N gene as the target regions to carry out the dual-target genes design. A one-step reverse transcription-polymerase chain reaction (RT-PCR) combined with Taqman hydrolysis probe technology was used to detect the RNAs in the sample by a fluorescence quantitative PCR instrument with changes in fluorescence signal.

The PCR detection system includes endogenous internal standard primers and probes and monitors the sample collection and extraction process by detecting whether the internal standard is normal to avoid false-negative results.

Applicable Instrument

ABI 7500™

Bio-Rad CFX96™PCR systems

Sample Requirements

1. Applicable sample types: upper respiratory tract samples (including throat swabs, nasal swabs, nasopharyngeal extracts, deep cough sputum); lower respiratory tract samples (including respiratory tract extracts, bronchial lavage fluid, lung tissue biopsy samples).

2. Sample collection: Collect according to the conventional sample collection method.

3. Sample storage and transportation: The collected specimens should be submitted for analysis in time, or stored at 4°C for 24 hours; it is best to store at -70°C for more than 24 hours and avoid repeated freeze-thaw cycles.

COVID-19 Lab Guidance & Diagnostics


  1. (2020) 2019-Novel coronavirus (2019-nCoV) real-time rRT-PCR panel primers and probes. US Centers for Disease Control and Prevention.

  2. (2020) 2019 Novel Coronavirus (2019-nCoV), Wuhan, China. [Online] US Centers for Disease Control and Prevention.

  3. (2020) Real-time RT-PCR panel for detection 2019-novel coronavirus. US Centers for Disease Control and Prevention. 

  4. (2020) Emergency use authorization. [Online] US Centers for Disease Control and Prevention.

Kits and reagents are sold for research use only. Not for use in diagnostic procedures.

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