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Automated Perfusion Systems Selection Guide

Pressure Controller and Peristaltic Perfusion Pump

PreciGenome offers a variety of perfusion systems to select based on different requirements of your applications. It includes pressure-driven perfusion systems and peristaltic perfusion pumps.   
 
These perfusion systems are convenient tools for a variety of applications, such as multi-reagent delivery, culturing cells at microscales, etc.
 
It allows control over microenvironmental cues, such as cell-cell and cell-matrix interactions; the potential to scale experiments; the use of small culture volumes; and the ability to integrate with microsystem technologies for on-chip experimentation.
 
It also allows precise and on-demand delivery and removal of biochemical reagent in the extracellular microenvironment, and controlled application of mechanical forces exerted via fluid flow.

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  • Drug screening

  • Toxicity tests

  • Calcium imaging

  • Bioreactor research

Option 1: 

Perfusion System with Single Reagent Delivery

 System Content:​

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  • PG-MFC-LT-2CH controller 1Pc

  • Reservoir Kit 2 Sets

  • Liquid flow sensor (optional)

  • Tubings and Fittings

 

Part #: PG-PF-MFL2-B

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Option 2: 

Perfusion System with Multiple Reagents Delivery

Many perfusion applications, such as organ-on-a-chip and cell culture, require switching between multiple reagents.  Combining PG-MFC pressure controller with a rotary valve, our system enables users to do multiple reagent switching. Our careful selected rotary valve has small internal channels (low dead volume) and an accurate positioning system which makes it ideal for precise liquid handling. Our system achieves fast switching without pushing a large amount of reagent to clean up the previous reagent during switching. Connected with a flow sensor, the system is capable of maintaining a constant flow rate through the feedback loop during the course of their experiments.

 System Benefits:​

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  • Automation

  • Fast switching time

  • Minimal cross-contamination between reagents

  • Pressure and flow-rate control

  • Easy setup and optimization

  • Low cost

  • OEM and custom design available

  • Temperature control module is also available to integrate into the system

System diagram of multi-reagent perfusion system. The system is able to run 2 independent experiments simultaneously. 
The video demonstrates multi-reagent switching with different colors using our system. The switching is fast and minimum cross-contamination.

 System Specs:​

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  • 2 Independent pressure channels

  • Max 2 sets of perfusion units running simultaneously

  • Reservoir volume: 15mL/50ml/1.5ml, larger volume options also available

  • Manifold: 10 to 1 Liquid flow rate: from 10nL/min to 5 ml/min

  • Dead volume: 4.5uL excluding the outlet tubing volume

 System Content:​

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  • PG-MFC-LT-2CH controller, 1PC (PG-MFC with touch screen is available to upgrade)

  • Rotary valve, 1to10 ports, 1PC

  • Reservoir kits (15mL, 50ml or 1.5ml reservoir volume), 5Sets

  • Liquid flow sensor (optional)

  • Tubings and Fittings

 

Part #: PG-PF-MFL2-MRD

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Applications:

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  • Droplets cell culture on the chip

  • Cell response to medium change

  • Live cell imaging

  • 3D cell culture

  • Stem cells assays

 

Option 3: 

Perfusion System with Reagent Recirculation

Combining our PG-MF controller with a 2-positions/6-ports valve, PreciGenome provides a perfusion system dedicated to cell culture in perfusion chambers with controlled shear force. This fully integrated solution includes all the necessary elements (hardware and software) to create a continuous and recirculated (unidirectional or bidirectional) flow and controlled flow rate (shear force) applied onto cells.

PG-MFC controller is used to provide pressure to pump reagent from reservoir 1 through liver-heart-on-a-chip to reservoir 2 through the valve. Combining PG-MFC pressure controller with one 2-positions/6-ports valve ( extra two 3-way valves required for light version controller), the system allows flowing buffers in two separate reservoirs back and forth, but still keeps the flow in the microfluidic chip uni-directional.

System Benefits:​

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  • Controlled shear stress

  • Long duration experiments

  • Automation

  • Controllable flow rates

  • Ready to connect with the incubator

  • Temperature control module is also available to integrate into the system

Work Modes:​

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  • Unidirectional flow control

  • Bidirectional flow control

  • Pulse flow control

3.1 Recirculation Perfusion System with PG-MFC Controller 

As showing in the schematics below, in position 1, reagent flows out from reservoir 1, through the 2 positions/6 ports valve, enters the microfluidic chip from its left side, and flows into reservoir 2. In this position, the 3-way valve on the left side connects pressure source to reservoir 1 and the other 3-way valve connects to the atmosphere.

 

In position 2, reagent flows out from reservoir 2, enters the microfluidic chip from its left side, and flows into reservoir 1. In this position, the 3-way valve on the right side connects pressure source to reservoir 2 and the other 3-way valve connects to the atmosphere. In this application, only a single pressure source is needed.

Example of microfluidic recirculation system setup for the organ-on-a-chip application. 
Unidirectional reagent flow is able to run through the chip over days.

 System Content:​

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  • PG-MFC controller, 1PC (4Channel , 8Channel or custumer version available)

  • Bidirectional 6-port / 2-position valve,  1PC

  • Reservoir Kit (15ml, 50ml or 100ml),  2Sets

  • Liquid flow sensor (optional)

  • Tubings and Fittings

 

Part #: PG-PF-MFC4-REC

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3.2 Recirculation Perfusion System          with Light Version Controller

 System Content:​

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  • PG-MFC-LT-2CH controller, 1PC 

  • Bidirectional 6-port / 2-position valve,  1PC

  • 3-way valves, 2PC

  • Reservoir Kit (15ml, 50ml or 100ml),  2Sets

  • Liquid flow sensor (optional)

  • Tubings and Fittings

 

Part #: PG-PF-MFL2-REC

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Multi-channel smart peristaltic pump is designed to meet your fluid transfer and dispensing needs with superior precision and accuracy, easy operation and minimum maintenance. The ultra-bright 4.3-inch LCD touch screen control panel interactively guides you at each step of the operation to set up sophisticated dispensing procedures in just seconds. In 4 different operation modes: Continuous Dispense Mode, Volume Dispense Mode, Repeat Dispense Mode, and Schedule Dispense Mode, the human-machine interface (HMI) accepts direct process parameter input and displays readout of rotor speed, flow rate, dispensed volume, remaining time or elapsed time data all on one screen.

Option 4: 

Perfusion System with Peristaltic Pump

System Benefits:​

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  • Long duration experiments

  • Automation

  • Controllable flow rates

  • Ready to connect with the incubator

  • Cost-effective