saCas9 mRNA LNP Premade Lipid Nanoparticle

Product Overview

Our saCas9 mRNA-LNP represents a cutting-edge gene editing delivery system designed for efficient transfection and in vivo delivery applications. This premade lipid nanoparticle formulation encapsulates wild-type Staphylococcus aureus Cas9 mRNA, enabling seamless cellular uptake and subsequent protein translation for precise gene editing applications.

Key Features

High-efficiency delivery: Optimally formulated LNPs ensure superior mRNA encapsulation and cellular uptake
Wild-type saCas9: Encodes fully functional Staphylococcus aureus Cas9 nuclease for robust gene editing
Versatile applications: Suitable for both in vitro transfection and in vivo delivery studies
Ready-to-use: Premade formulation eliminates preparation time and ensures batch-to-batch consistency
Optimized composition: Precisely balanced lipid ratios for maximum delivery efficiency

Mechanism of Action

Upon delivery, the saCas9 mRNA-LNP facilitates efficient cellular internalization through endocytosis. Following endosomal escape, the encapsulated mRNA is released into the cytoplasm where it undergoes translation to produce functional saCas9 protein. This nuclease can then be guided to specific genomic targets for precise gene editing applications.

Applications

mRNA delivery optimization studies
Translation efficiency analysis
Cell viability assessments
Gene editing research
In vivo delivery system development
Therapeutic mRNA research

Product Specifications

Payload:	saCas9 mRNA
Formulation:	Ionizable lipid, DSPC, PEG-2000 DMG, Cholesetrol
Storage Buffer:	10% Sucrose in TBS
Appearance:	Clear and colorless
Storage:	-20° C or below. We recommend to minimize freeze-thaw cycles to preserve LNPs integrity.
Shipping:	Dry ice
Size:	1 vial / pack. 100mg mRNA/vial
Concentration:	Batch specific (~0.1 mg/mL)
Stock:	On-demand

Storage and Handling

Storage Temperature: -20°C or below
Shipping: Dry ice
Stability: Minimize freeze-thaw cycles to preserve LNP integrity
Handling: Avoid repeated freeze-thaw cycles

Customization Options

Special formulations and custom requirements are available upon request to meet specific research needs.

Important Notes

For Research Use Only. Not for use in diagnostic procedures.

This product is exclusively designed for research applications and should be handled by qualified personnel familiar with molecular biology techniques and safety protocols.

For technical support, custom formulations, or bulk orders, please contact our scientific team.