纳米发电机™纳米粒子合成系统
Scalable throughput from microLiters to Liters
PreciGenome NanoGenerator™ 是用于纳米粒子合成的高性能仪器，如脂质纳米粒子、脂质体、PLGA 等，广泛用于药物输送、基因治疗、LNP 配制和制造等。
NanoGenerator™ 生成的纳米粒子具有更好的尺寸均匀性和更小的 PDI。它可从 0.1mL/样品筛选扩展到 1L（>10L 定制设计）大批量 GMP 生产
纳米粒子，尤其是脂质体和聚合物纳米粒子，由于其优异的性能，在药物递送、mRNA疫苗和生物传感等制药工业等各个领域显示出巨大的生物医学应用潜力。
通过微流控技术合成纳米颗粒比传统的批量合成工艺具有优势，因为它能够在尺寸和形状上具有更好的均匀性。例如，在药物递送领域，使用NanoGenerator™纳米粒子合成系统可以合成脂质纳米粒子（LNP）、脂质体、PLGA等多种纳米粒子。脂质纳米颗粒 (LNP)、脂质体和 PLGA 是最常用的可生物降解材料，用于输送亲水性和疏水性化合物。
粒子合成原理：
NanoGenerator™ 纳米粒子合成系统采用微流体装置进行可控和可调的聚合物粒子生产。下面的示意图说明了在聚焦流几何结构中为粒子合成而设计的结点装置。溶剂置换法用于纳米粒子合成。
纳米粒子合成：
聚合物流被挤压成彼此平行的窄流，与沿中央通道以较高流速流动的两个相邻水流平行，通过快速混合和自发扩散过程实现精确的纳米沉淀
系统优势：
High Performance & Efficiency
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Tunable size (40-200nm)
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Low PDI
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High encapsulation efficiency
Automation & QC
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Automated workflow
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Real-time data monitoring & recording
Open Platform
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LNP reagents
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Microfluidic mixer chips
Cost Effective
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Affordable system
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Low cost consumables
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Minimum maintenance
Scalable & Reproducible
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Scale-up and minimum process development
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Direct transfer from clinical development to commercial manufacturing
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Reproducible manufacturing
Custom Design & Service
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Custom design & OEM
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Research collaboration
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On-site 3Q installation & qualification (optional)
Path from Discovery to Commercialization
The NanoGenerator is a fully equipped instrument, ready for immediate use in the development of preclinical nanomedicine drugs. It offers controllable and reproducible mixing conditions, ensuring the accurate synthesis of LNPs through its scalable architecture found in the entire NanoGenerator product line.
NanoGenerator system offers controllable and reproducible mixing conditions, ensuring the accurate synthesis of LNPs through its scalable architecture found in the entire NanoGenerator product line. This architecture facilitates the seamless transfer of crucial process parameters, guaranteeing consistent critical quality attributes (CQAs) as one transitions from discovery and screening, process development to GMP production and commercial manufacturing for a wide range of applications, such as mRNA LNP vaccine development.
微流控混合器芯片:
PreciGenome 提供各种不同材料的微流控芯片，以满足我们大多数客户对纳米粒子合成的应用需求。
微流控混合器芯片通常用于纳米粒子合成。 三种类型的材料，包括聚合物、玻璃和硅，通常用于制造微流控芯片。芯片材料的选择取决于应用需求，包括芯片设计、实验所用溶剂或试剂的种类、应用需求、预算、制造时间等。
微流控方法的优点：
有效载荷：
mRNA/siRNA
Proteins and peptides
DNA
Small molecule drugs
系统内容：
纳米发电机 Flex
NanoGenerator™ Flex-S System facilitates the rapid screening of nanoparticle formulations and early-stage mRNA candidates, offering a substantial increase in project efficiency. It offers precise control of parameters ensures consistent critical quality attributes, guaranteeing speed, cost-effectiveness, and reliability at every stage.
Flex-S is designed for small scale production. The throughput volume range is from 0.1 to 0.5 mL, which is perfect for formulation screening and early discovery applications. Smaller output volume (<0.05ml per run) is achievable by recipe optimization. With smaller output volume, the reagents cost including payload and carrier can reduced more than 80% per run.
系统内容：
纳米发电机 Flex
NanoGenerator™ applies microfluidic approaches to synthesize nanoparticles (LNP, liposome, PLGA, etc.) in a continuous mode. NanoGenerator™ Flex-M system provides a wide throughput range from 1 to 12 mL, which meets a variety of applications from early screening to animal studies.
Flex-M system also provides an in-line dilution option to reduce ethanol concentration instantly. It further stabilizes LNP products.
系统内容：
纳米发电机 Flex
NanoGenerator™ Pro is an integrated nanoparticle synthesis system.
NanoGenerator™ Pro contains more powerful pressure control modules, which provide higher throughput from 2 to 200mL. The total flow rate range is from 4 to 20mL/min. The flow rate ratio (W:O) is between 2:1 to 5:1.
系统内容：
纳米发电机 Flex
NanoGenerator™ Max RUO version is widely used for preclinical application for lipid nanoparticle synthesis.
With PreciGenome microfluidics technology, customers can easily and seamlessly transfer their early discovery results (NanoGenerator Flex-S, Flex-M, and Pro) to late stage production (NanoGenerator Max cGMP).
NanoGenerator™ Max cGMP version is aslo available. It is designed for the clinical and commercial production.
微流控混合器芯片:
PreciGenome 提供各种不同材料的微流控芯片，以满足我们大多数客户对纳米粒子合成的应用需求。
微流控混合器芯片通常用于纳米粒子合成。 三种类型的材料，包括聚合物、玻璃和硅，通常用于制造微流控芯片。芯片材料的选择取决于应用需求，包括芯片设计、实验所用溶剂或试剂的种类、应用需求、预算、制造时间等。
LipidFlex™
LipidFlex™ 是一种三组分脂质混合物。
LipidFlex™ 可直接用于生成用于小分子封装的脂质体。
除了不同的阳离子/可电离脂质外，LipidFlex™ 还可用于生成用于核酸封装的脂质纳米颗粒 (LNP)。
特征：
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灵活的阳离子/可电离脂质比
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灵活多样的 N/P 比
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尺寸：80-120 nm synthesis
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PDI：0.05-0.2
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封装效率（EE%）：>90% （>95-99% w.优化条件）
综合服务：
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配方设计 (Lipid NP, Liposome or PLGA)
根据我们客户的需求，通过改变脂质成分、囊泡大小、表面电荷等来定制脂质体设计。
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载荷封装
定制方案，以高封装效率将药物分子封装到脂质纳米颗粒或 PLGA 中。
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细胞研究
细胞体外转染服务。
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分析和表征
对封装前后的脂质体进行综合分析，包括视觉外观、尺寸分布、稳定性、包封效率、封装效率分析、 体外释放曲线分析、释放速率等。
合成系统应用：
Gene Therapy
Genetic drugs, including small interfering RNA (siRNA), mRNA, or plasmid DNA, hold promise for treating a wide array of diseases by either suppressing harmful genes, producing therapeutic proteins, or employing gene-editing techniques. Currently, lipid nanoparticle (LNP) systems stand at the forefront among non-viral delivery methods, enabling the clinical utilization of genetic drugs.
Vaccines
Lipid nanoparticle has played a pivotal role in expediting the development of vaccines, as demonstrated by Pfizer and Moderna in the COVID-19 pandemic response. It allows for precise and high-throughput screening of potential vaccine candidates. The technology facilitates rapid testing, antigen formulation, and optimization of vaccine delivery systems.
Cell Therapy
Cell Therapies entail the transformation of cells, commonly immune cells, obtained either from the patient (autologous) or a compatible donor (allogeneic). These modified cells undergo isolation, amplification, and are later reintroduced into the patient. Lipid Nanoparticles (LNPs) present a versatile approach to cell reprogramming, facilitating the transfer of RNA responsible for protein expression or gene editing.
Other Areas
Other applications including cosmetics, medical imaging, nutrition, agrochemicals, etc.
The cosmetics sector stood at the forefront in acknowledging and utilizing nanotechnology advancements in diverse product innovations. Liposomal cosmetic formulations are expected to offer several benefits, including improved stability and effectiveness, along with successful ingredient penetration into the skin. A variety of liposomal cosmetics are currently in use.
演示视频和网络研讨会：
纳米发电机™Flex-M演示视频
纳米发电机™ProMab Biotechnologies 的 LNP 合成与应用网络研讨会
应用注意事项：
文件：
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NanoGenerator™ brochure
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NanoGenerator™ technical presentation
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Application notes for lipid nanoparticle synthesis
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Case study: T cell mRNA transfection
我们的一些客户
